Investigating Stress-relaxation and Failure Responses in the Trachea

Anita Singh; Tanmay Majmudar; Adi Iyer; Diya Iyer; Sriram Balasubramanian

doi:10.3791/64245

Investigating Stress-relaxation and Failure Responses in the Trachea

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Investigating Stress-relaxation and Failure Responses in the Trachea

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08:07 min

October 18, 2022

DOI: 10.3791/64245-v

Anita Singh¹, Tanmay Majmudar^2,3, Adi Iyer⁴, Diya Iyer⁴, Sriram Balasubramanian³

¹Department of Biomedical Engineering,Widener University, ²Drexel University College of Medicine, ³School of Biomedical Engineering, Science and Health Systems,Drexel University, ⁴Rosetree Media School District

Overview

This protocol offers a detailed approach to investigating the stress-relaxation responses of porcine tracheae, which is critical for understanding pulmonary mechanics. The methods described can enhance the understanding of the viscoelastic properties and failure thresholds of the trachea.

Key Study Components

Area of Science

Neuroscience
Biology
Pulmonary Mechanics

Background

The trachea plays a vital role in the respiratory system.
Understanding its mechanical properties is essential for pulmonary research.
Stress-relaxation responses provide insights into tissue behavior under stress.
Porcine models are commonly used in biomedical research due to anatomical similarities to humans.

Purpose of Study

To determine the tensile stress-relaxation and failure properties of porcine tracheae.
To improve understanding of the viscoelastic properties of the trachea.
To advance computational models of the pulmonary system.

Methods Used

Make a vertical midline incision along the neck of the cadaver.
Expose the thyroid cartilage, cricoid cartilage, and trachea.
Harvest the larynx and full length of the trachea.
Cut the trachea into strips for testing.

Main Results

Results provide insights into the viscoelastic behavior of the trachea.
Data can inform computational models of the pulmonary system.
Understanding failure thresholds can aid in clinical applications.
Findings contribute to the broader field of pulmonary mechanics research.

Conclusions

The protocol effectively assesses the mechanical properties of tracheal tissue.
Insights gained can enhance the understanding of respiratory mechanics.
Future research can build on these findings to improve pulmonary health.

Frequently Asked Questions

What is the significance of studying tracheal mechanics?

Studying tracheal mechanics helps understand respiratory function and can inform clinical practices.

How are porcine tracheae relevant to human health?

Porcine tracheae share anatomical similarities with human tracheae, making them suitable for research.

What methods are used to assess tracheal properties?

The protocol involves tensile stress-relaxation testing and mechanical property assessment.

Can this research impact computational modeling?

Yes, findings can enhance the accuracy of computational models of the pulmonary system.

What are the potential clinical applications of this research?

Understanding tracheal mechanics can improve treatments for respiratory conditions.

Is this protocol applicable to other species?

While focused on porcine tracheae, similar methods can be adapted for other species.

The present protocol determines the tensile stress-relaxation and failure properties of porcine tracheae. Results from such methods can help improve the understanding of the viscoelastic and failure thresholds of the trachea and help advance the capabilities of computational models of the pulmonary system.

This protocol offers a detailed approach to investigating the stress-relaxation responses of the trachea. Investigating the stress-relaxation responses of the trachea is critical to the understanding of pulmonary mechanics research. To begin, make a vertical midline incision along the neck of the cadaver and expose the thyroid cartilage, cricoid cartilage, and trachea from the hyoid bone to the suprasternal notch.

Harvest the larynx and the full length trachea using a 10 number blade. Separate the trachea sample from the larynx and then cut the tracheal tube longitudinally along the entire length on one side using the blade. Cut the trachea into two circumferential strips approximately five millimeter wide proximally and two longitudinal strips approximately five millimeter wide distally with the minimum length of these strips being 25 millimeters.

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