Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Summary

This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

Abstract

Synaptic terminals are the primary sites of neuronal communication. Synaptic dysfunction is a hallmark of many neuropsychiatric and neurological disorders. The characterization of synaptic sub-compartments by biochemical isolation is, therefore, a powerful method to elucidate the molecular bases of synaptic processes, both in health and disease. This protocol describes the isolation of synaptic terminals and synaptic sub-compartments from mouse brains by subcellular fractionation. First, sealed synaptic terminal structures, known as synaptosomes, are isolated following brain tissue homogenization. Synaptosomes are neuronal pre- and post-synaptic compartments with pinched-off and sealed membranes. These structures retain a metabolically active state and are valuable for studying synaptic structure and function. The synaptosomes are then subjected to hypotonic lysis and ultracentrifugation to obtain synaptic sub-compartments enriched for synaptic vesicles, synaptic cytosol, and synaptic plasma membrane. Fraction purity is confirmed by electron microscopy and biochemical enrichment analysis for proteins specific to sub-synaptic compartments. The presented method is a straightforward and valuable tool for studying the structural and functional characteristics of the synapse and the molecular etiology of various brain disorders.

Introduction

Synapses are the basic computational units of the brain through which neurons communicate and exert diverse and exquisitely complex functions. Synapses are, thus, fundamental to the health of the brain¹; synaptic dysfunction is implicated as a source or result of many disorders². Synapses are constituted by pre- and post-synaptic terminals, extensions of two different neurons that are closely apposed and separated by a synaptic cleft traversed by synaptic adhesion molecules. Information flows from the pre- to post-synaptic compartment in the form of chemical messengers called neurotransmitters¹. The molecular processes involved in neurotransmission are active areas of research^3,4,5. Understanding the pathogenic processes within synaptic terminals and the response of synapses to pathology in other neuronal sub-compartments are crucial steps to addressing disorders of the brain^1,2. Several methodological advancements, predominantly applied to murine models, have advanced this pursuit⁶. The isolation of synaptic fractions by differential centrifugation is one such paradigm-shifting method that has enabled the detailed evaluation of synaptic processes in health and disease.

The adult human brain consists of 80-90 billion neurons^7,8. Among murine species, the rat brain contains approximately ~200 million neurons, while mice have ~70 million^9,10. Each neuron forms thousands of specific synaptic connections with a network of highly polarized neurons intermingled with glial cells and dense vasculature. In such complex and heterogeneous tissue, it was once unthinkable to isolate and study synapses as an independent system. In the 1960s, Victor Whittaker, Catherine Hebb, and others made this possible by isolating intact synaptic terminals using subcellular fractionation^11,12,13,14. In an attempt to isolate synaptic vesicles (SVs), they homogenized brains through liquid shear force in iso-osmotic (0.32 M) sucrose followed by ultracentrifugation. They obtained pinched-off, plasma membrane-enclosed, intact nerve terminals or varicosities, which they called nerve-ending particles (NEPs)^11,13. As the structural and functional characteristics of the synapse were preserved in these structures, NEPs were later termed "synaptosomes" for congruence with other subcellular organelles^13,15. It is worth noting that the work of Eduardo de Robertis and colleagues, who coined the term "synaptic vesicle", overlapped with that of Whittaker and colleagues and contributed to the validation of "synaptosome" isolation and characterization^16,17,18.

Synaptosomes are physiologically active structures that contain all the cellular and molecular properties required for the storage, release, and reuptake of neurotransmitters^13,18. The preservation of key synaptic characteristics in vitro and freedom from non-synaptic components also contribute to the utility of this isolation method. Synaptosomes have contributed immensely to the understanding of the chemical and physiological properties of neurotransmission and are now being used to study synaptic molecular processes and their alterations in disease^{19,20,21,22,23}. Synaptosomes are also the initial source material for isolating synaptic components such as SVs, clathrin-coated vesicles (CCVs), synaptic cytosol, synaptic plasma membrane, synaptic mitochondria, synaptic adhesion molecules, and other components of interest, which can facilitate the understanding of the molecular mechanisms of synaptic function^{18,19,20,24,25,26,27,28}. These sub-synaptic components can be obtained by the osmotic lysis of synaptosomes and sucrose density gradient ultracentrifugation^15,29. Although the original subcellular fractionation method by Whittaker's research group is known to be efficient in isolating quality synaptosomes and SVs^13,30, recent optimizations enhance the purity of the subcellular fractions^22,23,31,32. This article provides a highly detailed and accessible version of a classic protocol for the subcellular fractionation of murine brain tissue to isolate synaptosomes, SVs, and other sub-synaptic components.

Protocol

All experiments with mice were approved by the Institutional Animal Care and Use Committee (IACUC) at Yale University (Protocol 2021-11117) and performed in a facility accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC). Animal care and housing complied with the Guide for the Care and Use of Laboratory Animals³³ and were provided by the Yale Animal Resource Center (YARC). Animals were maintained in a 12 h light/dark cycle with ad libitum access to food and water. Five to eight mice or two to four rats per genotype or condition are required for the following protocol. Fewer rats are necessary due to their larger brain volumes. Similarly, the age of the experimental animals may affect fraction yield; additional mice may be required for ages less than 2 months. Otherwise, the outlined procedures apply to both murine species and healthy adult animals of any age. The representative data presented in this study utilized wild-type (C57BL/6J) mice (age = 2 months; four males and four females per replicate) obtained from a commercial source (see Table of Materials).

1. Experimental preparation

NOTE: This protocol requires ~11 h for a single researcher to complete. It is highly recommended to complete benchtop setup (Figure 1), buffer preparation (Table 1), the precooling of centrifuges and rotors to 4 °C, and the collection and labeling of necessary materials and equipment (see Table of Materials) the day prior to protocol execution, where applicable.

Figure 1: Benchtop setup. Prior to brain dissections, (A) Dounce glass homogenizers and (B) all buffers were chilled on ice. (C) Protease inhibitor stock solutions were thawed on ice. A second container of wet ice for centrifuge tubes, a Dewar of liquid nitrogen (not shown), and (D) a container of dry ice for short-term storage of the samples flash-frozen in liquid nitrogen were obtained. (E) Microcentrifuge tubes were pre-labeled for all samples, as four aliquots of each subcellular fraction sample per genotype or condition were collected during this procedure (time-saving tip: thoroughly label all the tubes the day before the experiment is performed). (F) An appropriate biohazard waste container, (G) 70% ethanol, (H) surgical tools, and (I) an absorbent surface pad. The required centrifuge tubes and disposables were set aside for efficient access during protocol implementation (not shown). Please click here to view a larger version of this figure.

1. Prepare the benchtop for surgery and collect the scissors and forceps required for brain excision (see Table of Materials). Pre-label 1.5 mL microcentrifuge tubes for mouse tail biopsies and four tubes per collected fraction, as outlined in Figure 2.
2. Obtain two containers of wet ice, one container of dry ice, and a benchtop liquid nitrogen Dewar flask.
3. Thaw phenylmethylsulfonyl fluoride (PMSF), pepstatin A, aprotinin, and leupeptin stock solutions on ice (see Table of Materials). Prepare the necessary buffers (Table 1).
   NOTE: Sucrose solutions can be prepared in advance and stored at 4 °C. However, protease inhibitors (thawed stocks and tablets) must be added fresh to all the buffers at the start of the experiment due to the instability of these reagents in aqueous solutions. Further, all the buffers must be prepared with detergent-free glassware and detergent-free water to enable the collection of intact synaptosomes.
4. Chill all the buffers and glass Dounce homogenizers (see Table of Materials) on ice. Set the centrifuges to 4 °C and chill the rotors to 4 °C.
5. Add 14 mL of Buffer A (Table 1) to a Dounce homogenizer on ice.

Table 1: Composition of the subcellular fractionation buffers. Please click here to download this Table.

Figure 2: Overview of the subcellular fractionation protocol. Summary schematic of the subcellular fractionation steps and collected samples. Please click here to view a larger version of this figure.

2. Mouse brain excision

Figure 3: Craniofacial anatomy. (A) Dorsal view of a mouse skull with relevant cranial structures indicated. (B) Left lateral view of a mouse skull and brain with relevant cranial structures and anatomical directions indicated. The dashed lines represent the locations where incisions should be made. Please click here to view a larger version of this figure.

1. Deeply anesthetize each mouse with 100% isofluorane in an anesthesia chamber located in a fume hood or biosafety cabinet using an open drop method³⁴. Sacrifice each mouse by cervical spine dislocation followed swiftly by decapitation. Alternate between genotypes or experimental groups for each sacrifice and dissection¹². Obtain tail biopsies after euthanasia by excising 2 mm of the distal tail tip with fine scissors. Store the tissue for genotyping.
2. Spray the decapitated head with 70% ethanol to prevent hair from adhering to the tissue and surgical instruments during dissection.
3. Insert fine scissors under the skin at the decapitation incision to a pericranial depth and make a midsagittal incision up to the internasal suture (Figure 3A) to retract the scalp from the skull.
4. Working from the occipital area toward each temporal aspect, trim the fascia and muscle to expose the external surface of the skull beyond each external acoustic meatus (Figure 3B).
5. Secure the scalp and rostral aspect of the skull with the non-dominant hand. With the other, insert fine scissors 2 mm into the caudal side of the foramen magnum, where the spinal cord is visible exiting. Make a midline incision until the scissors reach the internal surface of the intraparietal bone (Figure 3; dashed lines).
   NOTE: During the initial incision, the scissors must be parallel to the spinal cord with pressure applied toward the internal surface of the skull to prevent damage to the brainstem and cerebellum.
6. Change the angle of the scissors so the blades run parallel with the dorsal surface of the skull. Continue advancing the midsagittal incision rostrally through the parietal and frontal bones, using the sagittal and interfrontal sutures as a guide. Use constant upward pressure to avoid damage to the cortex. Terminate the incision just beyond the internasal suture (Figure 3A).
7. Make a small perpendicular incision (~3 mm) to the nasal bone, rostral to the internasal suture, by placing the scissors perpendicular to the skull with each blade positioned at a nasal-premaxillary suture and making one even cut (Figure 3; dashed lines).
   NOTE: This step will increase the ease of retracting the skull and will be critical for collecting the olfactory bulb if this area is of interest.
8. While securing the rostral aspect, use one side of a pair of textured forceps to gently lift the skull up from the brain, then laterally and ventrally. Repeat along the midline as needed, then for the other hemisphere until the entire brain surface is exposed.
9. Using curved forceps or a fine spatula, gently lift the rostral side of the brain. Cut the optic and cranial nerves to complete the excision from the skull.
10. For each condition, collect five to eight mouse brains together into the chilled glass Dounce homogenizer containing 14 mL of Buffer A (Table 1).

3. Synaptosome preparation

NOTE: The schematics of this procedure are shown in Figure 4.

Figure 4: Synaptosome preparation. Schematic of step 3, the generation of synaptosomes (P2'). Please click here to view a larger version of this figure.

1. Homogenize the brains using a glass Dounce homogenizer in 12 up-down passes at 500 rpm (total). Pause briefly at each downstroke to ensure thorough homogenization of the tissue. Homogenize preferentially in an ice bath to avoid warming and protein denaturation. Take 5 µL aliquots for protein concentration determination by the bicinchoninic acid assay (BCA, see Table of Materials). Take 100 µL of whole brain lysate aliquots for western blot (WB). For this and all subsequent samples (Figure 2), take two aliquots for BCA and two aliquots for WB. Flash-freeze all the collected aliquots in liquid nitrogen and store them at −80 °C.
2. Spin the total brain homogenate in a high-speed round bottom centrifuge tube (14 mL) (see Table of Materials) at 800 x g for 10 min at 4 °C to obtain the supernatant (S1). Transfer S1 to a new centrifuge tube, leaving the pellet behind (P1), which contains intact cells and nuclei. Avoid pipetting up the fluffy, white, loose, superficial pellet. Take 2 x 5 µL of S1 for BCA and 2 x 100 µL of S1 for WB.
3. Spin S1 at 9,000 x g for 15 min at 4 °C to obtain the synaptosomal supernatant (S2) and crude synaptosome pellet (P2). Take 2 x 10 µL of S2 for BCA and 2 x 500 µL of S2 for WB. Discard the supernatant after obtaining aliquots and proceed to the next step with the pellet.
4. Resuspend P2 in 3 mL of ice-cold Buffer A with protease inhibitors and centrifuge at 9,000 x g for 15 min at 4 °C to obtain the supernatant (S2') and washed synaptosomes (P2'). Discard the supernatant and keep the pellet.
5. Resuspend P2' in 3 mL of Buffer A. Avoid resuspending the dark red portion at the bottom of the pellet, which mainly contains mitochondria. Take 2 x 20 µL of P2' for BCA and 2 x 100 µL of P2' for WB.
   NOTE: This can be achieved by gently pipette-mixing the edges and surface of the pellet to resuspend the white washed synaptosomes while directing the pipette tip away from the red center of the pellet.

4. Hypotonic lysis

NOTE: The schematics of this procedure are shown in Figure 5.

Figure 5: Hypotonic lysis. Schematic of step 4, the hypotonic lysis of synaptosomes to generate the lysis supernatant (LS1) and synaptosomal membrane fractions (LP1). Please click here to view a larger version of this figure.

1. For the hypotonic lysis of washed synaptosomes, add 9 volumes of chilled Buffer B (Table 1) to resuspended P2' (~27 mL). Homogenize the synaptosomes in a glass Dounce homogenizer (three up-down passes at 500 rpm).
2. Transfer the samples to 50 mL capped conical centrifuge tubes. Rotate them on a tube revolver in a 4 °C cold room for 15 min.
3. Centrifuge lysed P2' at 25,000 x g for 20 min at 4 °C to obtain the lysis supernatant (LS1) and the lysis pellet containing synaptosomal membranes (LP1). Take 2 x 50 µL of LS1 for BCA and 2 x 400 µL of LS1 for WB. Transfer LS1 into a capped centrifuge tube for ultracentrifugation (see Table of Materials).

5. Synaptic vesicle isolation

NOTE: The schematics of this procedure are shown in Figure 6.

Figure 6: Synaptic vesicle isolation and synaptic plasma membrane isolation. (A) Schematic of step 5, the isolation of synaptic cytosol (LS2) and synaptic vesicle (LP2) fractions, and (B) step 6, the generation of myelin (MF), synaptic plasma membrane (SPM), and mitochondrial (Mito.) fractions following the ultracentrifugation of sucrose gradients. Please click here to view a larger version of this figure.

1. Centrifuge LS1 in a fixed angle ultracentrifuge rotor (see Table of Materials) at 100,000 x g for 60 min at 4 °C to obtain synaptic cytosol supernatant (LS2) and synaptic vesicle pellet (LP2). LP2 will be small, translucent, and strongly adhered to the side of the centrifuge tube.
2. Resuspend LP2 in 500 µL of Buffer A. Using a 23 G needle and a 1 mL syringe, shear LP2 with gentle trituration. Take 2 x 10 µL of LP2 for BCA and 2 x 250 µL of LP2 for WB.
3. Transfer LS2 (~30 mL) to centrifugal filter units with a 10 kDa cutoff (see Table of Materials).
   NOTE: If proteins smaller than 10 kDa are of interest, 4 kDa cutoff centrifugal filter units are available but will result in longer spin times.
4. Concentrate LS2 to approximately 0.5 mL by spinning at 5000 x g for up to 1 h at 4 °C. Take 2 x 10 µL of concentrated LS2 for BCA and 2 x 250 µL of concentrated LS2 for WB. After starting the spin, proceed directly to step 6.1.

6. Synaptic plasma membrane isolation

1. Resuspend LP1 (step 4.3) in 1 mL of Buffer B (Table 1). Take 2 x 10 µL of LP1 for BCA and 2 x 50 µL of LP1 for WB. Adjust the remaining LP1 to a final volume of 7.5 mL and a final sucrose concentration of 1.1 M with Buffer B and Buffer C (Table 1).
2. Transfer 7.5 mL of resuspended LP1 into a 14 mL ultracentrifuge tube (see Table of Materials). Carefully overlay LP1 with 3.75 mL of Buffer D (Table 1), and then overlay with 1.25 mL of Buffer A (or a larger volume to fill just below the top of the centrifuge tube). Avoid pipetting down the side of the tube, which will disrupt the sucrose gradient interfaces. After overlaying each sucrose fraction, mark the top of the solution with a pen. Balance the tubes for ultracentrifugation by weight, not volume, with the dropwise addition of Buffer A to within 10 mg. Centrifuge at 48,000 x g for 2.5 h at 4 °C in a swinging bucket ultracentrifuge rotor (see Table of Materials).
3. Acquire images of the intact gradients following ultracentrifugation to document the distinctness of each sucrose interface and the success of fractionation.
4. Carefully remove the superficial layer of 320 mM sucrose (Buffer A). Recover the myelin fraction (MF) at the 320 mM/855 mM sucrose interface in an 800 µL volume. Recover the synaptic plasma membrane (SPM) fraction at the 855 mM/1.1 M sucrose interface in a 1,000 µL volume. Pipette each fraction up from the wall of the tube in a circular manner to ensure the complete fraction is collected. Carefully aspirate off the remaining sucrose and recover the mitochondrial pellet (Mito.) by resuspending in 200 µL of Buffer B. Take 2 x 100 µL of MF for BCA and 2 x 10 µL of Mito. for BCA; divide the remainder of MF and Mito. samples in half for WB.
5. Dilute the SPM fraction with 2 volumes of Buffer B (~2 mL), and then centrifuge in a fixed angle rotor in a 3.5 mL centrifuge tube (see Table of Materials) at 25,000 x g for 20 min at 4 °C. Discard the supernatant and resuspend the SPM pellet in Buffer A for a final volume of 250 µL. Take 2 x 5 µL of SPM for BCA and divide the remaining SPM in half for WB.
6. Perform a BCA to determine the protein concentration of each sample, accounting for variable aliquot volume.
   NOTE: For WB analysis, the suggested working protein concentration for all subcellular fractions is 2 µg/µL (or as high as achievable for LS1 and MF).

Results

The presented method results in 11 brain subcellular fractions that can be subjected to further purification and various forms of downstream analysis^35,36. The gold standard method to assess the quality of synaptosomes, SVs²³, and other components is electron microscopy (EM) (Figure 7). Quantitative immunoblotting for proteins that are present in specific subcellular fractions can also be performed to assess markers of fraction purity (Figure 8). For example, immunoblot analysis of fractions reveals the enrichment of N-cadherin (CDH2, UniProt name) in the synaptic plasma membrane fraction (SPM), α-synuclein (SYUA) in the synaptic cytosol (LS2), synaptophysin (SYPH) in the synaptic vesicle fraction (LP2), and myelin basic protein (MBP) in the myelin fraction (MF) when compared to protein levels in the initial whole brain homogenate (Total) (Figure 8). Once fraction purity has been established (for example, note the absence of CDH2 in the LS2 fraction or the many-fold increase in SYPH in the LP2 fraction), quantitative immunoblotting can be used to determine the localization of proteins of interest or query differences in protein distribution between genotypes or treatments. Understanding the subcellular localization of synaptic proteins can enable the dissection of previously undescribed protein functions. Further, this method may elucidate trafficking defects or synaptic dysfunction in disease states, especially when paired with functional assays. For example, our team has used this method to identify a pool of enzymatically active palmitoyl protein thioesterase 1 that is enriched in the synaptic cytosol¹⁹.

Figure 7: Electron microscopy (EM) of synaptosomes. (A) Representative EM image of a synaptosome containing synaptic vesicles (arrow). (B) Representative EM image of a synaptosome with both pre- (arrow) and post-synaptic components (double arrow). (C) Representative EM image of a synaptosome containing synaptic vesicles and a mitochondrion (arrow) (scale bars = 100 nm). Please click here to view a larger version of this figure.

Figure 8: Immunoblot analysis of subcellular fractions. (A) Markers of subcellular fraction purity (indicated with UniProt nomenclature) are appropriately localized compared to the whole brain homogenate (total): N-cadherin (CDH2) in the synaptic plasma membrane fraction (SPM), synaptophysin 1 (SYPH) and synaptojanin 1 (SYNJ1) in the synaptic vesicle enriched fraction (LP2), α-synuclein (SYUA) in the synaptic cytosol (LS2), and myelin basic protein (MBP) in the myelin fraction (MF). (B) Immunoblot quantification analysis reveals the enrichment (fold-change from total) of fraction purity markers. Data are represented as mean ± standard deviation on a log10 scale. The dotted line indicates a 1.5-fold change (y = 0.176) (n = 3 replicate experiments with 8 wild-type mice; age = 2 months; n = 4-5 blots for SYPH, SYUA, MBP, with n = 3 plotted values previously published by Gorenberg et al.¹⁹; n = 5 for SYNJ1; n = 1 for CDH2). Please click here to view a larger version of this figure.

Discussion

In their seminal studies, Whittaker and colleagues used four morphological criteria to identify synaptosomes: (1) the structures have a sealed plasma membrane; (2) the structures contain SVs resembling those in nerve terminals and varicosities in situ in size and number; (3) the structures possess one or more small mitochondria; and (4) the presynaptic membrane is frequently adhered to a post-synaptic component^11,12,13. Though the first two criteria generally apply to every isolation method, in the most recent protocols described in this article, not all resulting synaptosomes will have mitochondria and attached post-synaptic terminals. Approximately 60% of the synaptosomes will have mitochondria, and only up to 15% are estimated to have attached post-synaptic terminals³⁷. If post-synaptic components are of particular interest, the use of an isotonic Krebs-like homogenization buffer and pressure filtration for enrichment are known to yield high concentrations of synaptosomes with post-synaptic terminals (also termed synaptoneurosomes)^22,38.

The method of sacrificing the animal can impact the quality of synaptosomes and synaptic subfractions. Adult animals sacrificed using a euthanasia method that does not require anesthesia will result in the best fraction quality. Further, the brains should be freshly dissected, not frozen, and homogenized using a 1:10 ratio of homogenization buffer (weight/volume) for the most viable synaptic fractions²². The brain has a heterogeneous population of synapses that can be differentiated by the type of neurotransmitters they carry. Synaptosome formation is generally unaffected by synapse type or neurotransmitter content¹³. An exception is mossy fibers in the cerebellum, which are known to be disrupted in optimal conditions for obtaining synaptosomes from the rest of the brain^39,40. Thus, removal of the cerebellum prior to brain homogenization is recommended if the exclusion of this region does not affect the experimental goal. If interested in isolating synaptosomes of a particular neurotransmitter character, areas of the brain that are enriched for neurons containing the neurotransmitter of interest can first be isolated. However, this approach will impose limitations on the final fraction yield, depending on the size of the region of interest (the age of animals is also, therefore, a consideration). There are immunochemical methods for the isolation of neurotransmitter-specific synaptosomes, but the viability and yield will be significantly compromised²². If assessing synaptosome metabolic viability is important, the measurement of neurotransmitter release^41,42 or certain enzymatic assays⁴³ can be employed.

Common contaminants in synaptosome preparations include microsomes, free mitochondria, SVs, and neuronal and glial membranes. Contamination can be reduced by increasing the number of washes at the P1 and P2 fractions²² and avoiding resuspension of the red mitochondrial pellet in subsequent steps. In experiments where metabolic viability and time are crucial, reducing the number of washes and using Ficoll or Percoll gradients over sucrose gradients will be helpful^44,45,46. These methods also reduce contamination significantly. Whittaker's original protocol yielded high-quality SVs. Further optimization by Nagy et al.²³, included in this method, produces SVs with remarkable homogeneity and purity without compromising significantly on yield³⁶. If specific SV subtypes are of interest, such as glutamatergic (VGLUT-1-containing) or GABAergic (VGAT-1-containing) SVs, immunoisolation using specific antibodies can be performed^47,48. Alternative methods are also available for isolating CCVs from synaptosomes, which, due to differential density, may not be present at the same interface as SVs obtained with this method^20,49,50.

Overall, the present protocol to isolate synaptic components can be further optimized to obtain fractions with improved homogeneity and viability based on the quality and quantity of the source brain tissue and the experimental goals. For further troubleshooting details, one should refer to book chapters by Dunkley and Robinson²² and Ganzella et al.³⁶.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
1 mL TB Syringe	BD	309649
1.5 mL Eppendorf Tubes	USA Scientific	1415-2500
14 mL, Open-Top Thinwall Ultra-Clear Tube	Beckman Coulter	344060	Compatible with SW 40 Ti
23 Gauge Precision Glide Hypodermic Needle	BD	305145
26.3 mL, Polycarbonate Bottle with Cap Assembly	Beckman Coulter	355618	Compatible with Ti70
3.5 mL, Open-Top Thickwall Polypropylene Tube	Beckman Coulter	349623	Compatible with TLA-100.3
50 mL Falcon Tubes	Fisher Scientific	14-432-22
Amicon Ultra-15 Centrifugal Filter Unit	Millipore Sigma	UFC901024
Aprotinin	Sigma-Aldrich	A6279	1 mg/mL in diH2O
Avanti J-26 XP Centrifuge	Beckman Coulter	B22984	<26,000 rpm
Benchtop HDPE Dewar Flask	Thermo Scientific	5028U19
C57BL/6J Mice	The Jackson Labs	000664
Centrifuge 5810R	Eppendorf	EP022628168	<14,000 rpm
complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets	Roche	11873580001	Add 1 tablet per 50 mL of solution
Curved Forceps	Fine Science Tools	11273-20
Fine Surgical Scissors	Fine Science Tools	8r
Glas-Col Tissue Homogenizing System	Cole-Parmer	UX-04369-15
Graefe Forceps	Fine Science Tools	11650-10
High-Speed Polycarbonate Round Bottom Centrifuge Tubes	ThermoFisher	3117-0500	Compatible with JA20
Isofluorane	Henry Schein Animal Health	NDC 11695-6776-2
JA-20 Rotor	Beckman Coulter	334831
Leupeptin	American Bio	AB01108	1 mg/mL in diH2O
N-[2-Hydroxyethyl] piperazine-N’-[2-ethanesulfonic acid] (HEPES)	American Bio	AB00892
Optima L-80 XP Ultracentrifuge	Beckman Coulter		<100,000 rpm
Optima TLX Ultracentrifuge	Beckman Coulter		<120,000 rpm
Pepstatin A	Thermo Scientific	78436	1 mg/mL in DMSO
Phenylmethylsulfonyl fluoride (PMSF)	American Bio	AB01620
Pierce BCA Protein Assay Kit	Thermo Scientific	23335	For determination of protein concentration
Pipette Tips
Serological Pipettes
Sucrose	Sigma-Aldrich	S0389
Surgical Scissors	Fine Science Tools	14002-12
SW 40 Ti Swinging-Bucket Rotor	Beckman Coulter	331301
Teflon-Coated Pestle and Mortar Tissue Grinder	Thomas Scientific	3431D94
Ti70 Rotor	Beckman Coulter	337922
TLA-100.3 Rotor	Beckman Coulter	349490
Tube Revolver	Dot Scientific	DTR-02VS