Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Haseong Kim; Kil Koang Kwon; Wonjae Seong; Seung-Goo Lee

doi:10.3791/54059

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Çok enzim Yüksek verim Genetik Enzim Eleme Sisteminin Kullanılması Eleme

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JoVE Journal Biyoloji

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Çok enzim Yüksek verim Genetik Enzim Eleme Sisteminin Kullanılması Eleme

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8,644 Views

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08:10 min

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August 08, 2016

DOI:

10.3791/54059-v

DOI:

10.3791/54059-v

•

08:10 min

August 08, 2016

•

5 Views

Haseong Kim¹, Kil Koang Kwon¹, Wonjae Seong¹, Seung-Goo Lee^1,2

¹Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, ²Biosystems and Bioengineering Program,University of Science and Technology, Daejeon, South Korea

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Özet

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).