Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Yeri Alice Rim; Yoojun Nam; Ji Hyeon Ju

doi:10.3791/54650

Dergi

/

Gelişim Biyolojisi

/

Sendai Virüs ve santrifüj kullanılarak Kan Hücreleri indüklenmiş pluripotent kök hücre Üretimi

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

JoVE Journal
Gelişim Biyolojisi

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal Gelişim Biyolojisi

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Sendai Virüs ve santrifüj kullanılarak Kan Hücreleri indüklenmiş pluripotent kök hücre Üretimi

Please note that all translations are automatically generated. Click here for the English version.

14,177 Views

•

09:57 min

•

December 21, 2016

DOI:

10.3791/54650-v

DOI:

10.3791/54650-v

•

09:57 min

December 21, 2016

•

12 Views

Yeri Alice Rim¹, Yoojun Nam¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine,The Catholic University of Korea

DEŞİFRE METNİ

Özet

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.