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Kütle spektrometresi tabanlı kimlik RNA-bağlama bölgelerinin için numune hazırlama
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Kütle spektrometresi tabanlı kimlik RNA-bağlama bölgelerinin için numune hazırlama

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8,091 Views

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10:52 min

•

September 28, 2017

DOI:

10.3791/56004-v

DOI:
10.3791/56004-v

•

10:52 min
September 28, 2017

•

8066 Views
, , , ,
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

Özet

Automatically generated

Biz RNA bağlanıcı proteinler tanımlamak ve kendi RNA bağlayıcı bölgeleri UV-aracılı photocrosslinking ve kütle spektrometresi kullanılarak canlı hücrelerdeki eşlemek için bir protokol tanımlamak.

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Cite this Article

Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).

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