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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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JoVE Journal Biology
Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

DOI:
10.3791/62878-v

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06:58 min

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October 07, 2021

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, ,
1Faculty of Biology,Technion – Israel Institute of Technology

Chapters

  • 00:04Introduction
  • 00:49Cell Collection and Lysis
  • 01:29Processing the Total RNA Sample
  • 02:24Processing the Immunopurification Sample
  • 04:34Results: Analysis of Ribosome-Protected Footprint Isolation, RNA Extraction, cDNA Library Creation, and Co-Translational Interactions
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

Co-translational interactions play a crucial role in nascent-chain modifications, targeting, folding, and assembly pathways. Here, we describe Selective Ribosome Profiling, a method for in vivo, direct analysis of these interactions in the model eukaryote Saccharomyces cerevisiae.

Tags

Keywords: Co-translational InteractionsSelective Ribosome ProfilingIn Vivo AnalysisSaccaromyces CerevisiaeCryogenic GrindingRNase I DigestionSucrose CushionAffinity PurificationProtein-ribosome InteractionsGlobal Profiling

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