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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
 

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Article DOI: 10.3791/63366-v
February 18th, 2022

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Summary February 18th, 2022

Translating ribosomes decode three nucleotides per codon into peptides. Their movement along mRNA, captured by ribosome profiling, produces the footprints exhibiting characteristic triplet periodicity. This protocol describes how to use RiboCode to decipher this prominent feature from ribosome profiling data to identify actively translated open reading frames at the whole-transcriptome level.

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