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Biology
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用于测量的活性测定
大肠埃希氏菌
诱导赖氨酸Decarboxyase
JoVE Journal
Biology
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JoVE Journal
Biology
An Assay for Measuring the Activity of
Escherichia coli
Inducible Lysine Decarboxyase
Please note that all translations are automatically generated.
Click here for the English version.
用于测量的活性测定
大肠埃希氏菌
诱导赖氨酸Decarboxyase
DOI:
10.3791/2094-v
•
08:08 min
•
December 19, 2010
•
Usheer Kanjee
,
Walid A. Houry
1
Department of Biochemistry
,
University of Toronto
Chapters
00:05
Title
01:10
Introduction
01:39
Enzyme Assay Preparation
02:51
Lysine Decarboxylase (Ldcl) Enzyme Assay
04:17
Trinitrobenzene Sulfonic Acid (TNBS) Reaction and Color Development
06:42
Ldcl Assay Representative Results
07:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
2,4,6 - trinitrobenzensulfonic酸反应的底物L -赖氨酸和产品尸形成加合物在甲苯的溶解度差,诱导赖氨酸脱羧酶的活动都受到监控。
Tags
Assay
Measuring
Activity
Escherichia Coli
Inducible Lysine Decarboxyase
PH
Acidic PH Survival
Amino Acid Decarboxylases
Glutamic Acid Decarboxylases
Arginine Decarboxylase
Lysine Decarboxylase
Ornithine Decarboxylase
Pyridoxal-5′-phosphate
Co-factor
Inner-membrane Substrate-product Antiporters
Lysine-cadaverine Antiporter
CadB
X-ray Crystal Structure
Stringent Response Regulator Guanosine 5′-diphosphate 3′-diphosphate
PpGpp
Stringent Response
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