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Etkinlik Ölçüm Bir Testi Escherichia coli Lizin Decarboxyase Đndüklenebilir
JoVE Journal
Biology
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JoVE Journal Biology
An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
DOI:

08:08 min

December 19, 2010

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Chapters

  • 00:05Title
  • 01:10Introduction
  • 01:39Enzyme Assay Preparation
  • 02:51Lysine Decarboxylase (Ldcl) Enzyme Assay
  • 04:17Trinitrobenzene Sulfonic Acid (TNBS) Reaction and Color Development
  • 06:42Ldcl Assay Representative Results
  • 07:31Conclusion

Summary

Automatic Translation

Indüklenebilir lizin dekarboksilaz aktivitesi toluen diferansiyel çözünürlüğü adducts oluşturmak için substrat L-lizin ve ürün cadaverine 2,4,6-trinitrobenzensulfonic asit ile reaksiyona girerek izlenir.

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