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Journal / Immunology and Infection / Marcado específico del VIH-1 células positivas…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Marcado específico del VIH-1 células positivas utilizando un vector lentiviral Ap-dependientes que expresan la proteína verde fluorescente
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Marcado específico del VIH-1 células positivas utilizando un vector lentiviral Ap-dependientes que expresan la proteína verde fluorescente

Article DOI: 10.3791/2198-v • 09:54 min • September 23rd, 2010
September 23rd, 2010 •
*1, *1, 1
1National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University
* These authors contributed equally

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Hemos desarrollado un vector lentiviral que posee, además de la LTR-Tat respuesta, el elemento de respuesta Rev (RRE) que pueden regular la expresión del gen de una forma del VIH-1 Tat y Rev-dependiente. El vector permite la detección específica de la replicación del VIH en las células vivas a través de la expresión de GFP.

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Enfermedades Infecciosas Número 43 del VIH-1 Ap GFP lentiviral vector RRE
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