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An Introduction to Worm Lab
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An Introduction to Worm Lab: from Culturing Worms to Mutagenesis

Article DOI: 10.3791/2293-v • 10:44 min • January 11th, 2011
January 11th, 2011 •
*1, *1, 1
1Department of Biology, University of Texas at Arlington
* These authors contributed equally

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Screening for mutants with phenotypic defects is a straightforward method for identifying genes that function in a given biological process. In this article we describe how to culture free living worms (e.g., Pristionchus pacificus) in the laboratory and show two different mutagenesis methods, EMS and TMP/UV.

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Worm Lab Culturing Worms Mutagenesis Nematodes Laboratory Maintenance Ethyl Methane Sulfonate EMS 4 5' 8-trimethylpsoralen TMP/UV Genetics Studies Self-fertilizing Hermaphrodites Mating System Agar Plates Bacterial Lawn Point Mutations Small Deletions Recessive Mutations Pristionchus Pacificus Phenotypes Uncoordinated Mutants Dumpy Mutants Transformer Mutants
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