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Journal
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Immunology and Infection
/
胸腺淋巴瘤的T细胞系的推导
ATM
- / -</sup
P53
- / -</sup
鼠标
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Derivation of Thymic Lymphoma T-cell Lines from
Atm
-/-
and
p53
-/-
Mice
Please note that all translations are automatically generated.
Click here for the English version.
胸腺淋巴瘤的T细胞系的推导
ATM
- / -</sup
P53
- / -</sup
鼠标
DOI:
10.3791/2598-v
•
17:59 min
•
April 03, 2011
•
Rasika Jinadasa
,
Gabriel Balmus
,
Lee Gerwitz
,
Jamie Roden
,
Robert Weiss
,
Gerald Duhamel
1
Department of Biomedical Sciences
,
Cornell University
Chapters
00:01
Title
00:05
Introduction
00:34
Dissection of Tumor
04:11
Culturing Lymphoma Cells
10:33
Feeding the Cells
11:36
Passaging the Cells
11:44
Initial Passage
15:17
Maintaining Established Cell Lines
16:42
Freezing and Recovering the Cell Lines
17:33
Cell Recovery from Liquid Nitrogen
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频中,我们展示了一个协议来建立小鼠胸腺淋巴瘤细胞株。通过这个协议,我们已经成功地建立了几个从ATM / T细胞线 - 和p53 - / - 小鼠胸腺淋巴瘤。
Tags
Thymic Lymphoma
T-cell Lines
Atm-/- Mice
P53-/- Mice
Genetically Modified Mice
Laboratory Animals
Research Tool
Murine T-cell Lines
Interleukins
Protocol
Tumors
Visible Tumors
Hunched Posture
Labored Breathing
Poor Grooming
Wasting
Susceptible Strain
Inbred Strains
CD3 Expression
CD4 Expression
CD8 Expression
Passaged
Article
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