Neuroscience
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Alta sensibilidad de detección de 5 hidroximetilcitosina en el tejido cerebral Balb / C
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Summary February 1st, 2011
Please note that all translations are automatically generated.
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El EpiMark 5 HMC y 5-MC Kit de análisis se puede utilizar para analizar y cuantificar la 5-metilcitosina y 5 hidroximetilcitosina dentro de un lugar específico de la SPE. El kit distingue 5-MC de 5 HMC por la adición de glucosa al grupo hidroxilo de la 5-HMC a través de una reacción enzimática utilizando β-glucosiltransferasa (T4-BGT). Cuando el 5-HMC se produce en el contexto de las CCGG, esta modificación convierte un sitio escindible MspI a un sitio no escindibles.
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