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Mammary Transplantation von Stromazellen und Carcinoma Cells in C57BL/6J-Mäuse
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Medicine
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JoVE Journal Medicine
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
DOI:

13:01 min

August 12, 2011

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Chapters

  • 00:05Title
  • 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
  • 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
  • 06:33Preparation of Collagen Embedded Cells for Grafting
  • 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
  • 10:40Representative Results

Summary

Automatic Translation

In diesem Bericht zeigen wir ein System zur Isolierung und Kultur Spenderzellen von der Maus Brustdrüse und orthotop Transplantation dieser Zellen in Empfängermäuse zu Stromatumoren analysieren: epitheliale Interaktionen während Mammatumor Entwicklung.

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