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植入前胚胎,牛,羊,山羊的冷冻保存
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Please note that all translations are automatically generated.
Click here for the English version.
植入前胚胎,牛,羊,山羊的冷冻保存
DOI:
10.3791/2764-v
•
11:10 min
•
August 05, 2011
•
Curtis R. Youngs
1
Animal Science Department
,
Iowa State University
Chapters
00:05
Title
02:28
Evaluating Suitability of Embryos for Cryopreservation
03:58
Washing Embryos to Reduce Likelihood of Disease Transmission
05:20
Dehydrating Embryos and Loading Embryos into a 0.25 ml Plastic Straw
07:11
Freezing Embryos
08:53
Thawing Cryopreserved Embryos
10:26
Results of Transferring Frozen-thawed Embryos to Female Recipients
10:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
植入前胚胎可冷冻保存,放置到一个高渗cryoprotective溶液后,引起细胞脱水。平衡后,冰晶体的形成是诱导胚胎周围的解决方案。进一步脱水发生胚胎慢慢冷却到零度以下的温度中,前陷入液氮存储。
Tags
Cryopreservation
Preimplantation Embryos
Cattle
Sheep
Goats
Cryoprotective Agent
Ethylene Glycol
Glycerol
Osmotic Gradient
Cellular Dehydration
Controlled Rate Freezer
Ice Crystal Formation
Liquid Nitrogen
Thawing
Recipient Female
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