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In-vivo Centrifugation of Drosophila Embryos

In-vivo Centrifugation of Drosophila Embryos

Article DOI: 10.3791/2005-v 13:55 min June 23rd, 2010
June 23rd, 2010



We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.


In-vivo Centrifugation Drosophila Embryos Organelle Separation Buoyant Density Intact Embryos Living Embryos Agar Plate Vertical Holes Centrifugation Stratification Bright-field Microscopy Fluorescent Markers Colocalization Biochemical Analysis Transplantation
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