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Chapters
- 00:00Title
- 00:43Introduction
- 01:38Preparing Apple Juice Agar Plates and Harvesting Embryos
- 03:26Removing the Egg Shell From the Embryos (Dechorionation)
- 05:09Mounting the Embroys in Agar
- 08:28Centrifugation and Recovery of Embryos, Followed by Further Processing
- 13:00Conclusion
We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.
