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JoVE Journal
Biology

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In-vivo Centrifugation of Drosophila Embryos
 

In-vivo Centrifugation of Drosophila Embryos

Article doi: 10.3791/2005
June 23rd, 2010

Summary June 23rd, 2010

We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.

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