8,761 Views
•
13:07 min
•
July 04, 2007
DOI:
Summary
Automatically generated
This video demonstrates the preparation of primary neuronal cultures from midgastrula stage Drosophila embryos. Views of live cultures show cells 1 hour after plating and differentiated neurons after 2 days of growth in a bicarbonate-based defined medium. The neurons are electrically excitable and form synaptic connections.
Explore More Videos
Related Videos
08:24
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Related Videos
17671 Views
15:12
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
Related Videos
15008 Views
08:45
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
Related Videos
13867 Views
10:55
Primary Cell Cultures from Drosophila Gastrula Embryos
Related Videos
13825 Views
11:56
Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
Related Videos
7731 Views
10:08
Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.
Related Videos
13658 Views
05:54
Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants
Related Videos
2975 Views
01:17
An Indirect Neuron-Astrocyte Co-Culture Technique for Studying Neuron-Glia Interactions
Related Videos
178 Views
01:19
Analyzing Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants
Related Videos
22 Views
01:21
Culturing and Maintaining Dopaminergic Neurons from Mouse Embryonic Brain Tissue
Related Videos
45 Views
Read Article
Cite this Article
Sicaeros, B., O'Dowd, D. K. Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos. J. Vis. Exp. (5), e226, doi:10.3791/226 (2007).
Copy