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Chapters
- 00:00Introduction
- 01:35Embryo Dechorionation with Bleach
- 02:44Preparation of Single Embryo Cultures
- 07:08Obtaining Single Midgastrula Stage Embryo Cultures
- 08:48Maintaining Healthy Embryonic Cultures
- 10:09Viewing Embryonic Cultures
- 12:09Conclusion
- 12:52End Credits
This video demonstrates the preparation of primary neuronal cultures from midgastrula stage Drosophila embryos. Views of live cultures show cells 1 hour after plating and differentiated neurons after 2 days of growth in a bicarbonate-based defined medium. The neurons are electrically excitable and form synaptic connections.
Tags
Neuronal CulturesMidgastrula StageDrosophila EmbryosPrimary Neuronal CulturesEmbryo CollectionDechorionationCell RemovalCell DispersionMedium DropUncoated Glass CoverslipCell DensityNeuroblastsNeuritic ProcessesNeurite OutgrowthBranchingDefined MediumCO2 IncubatorSynaptic ConnectionsVoltage-gated Sodium ChannelsCalcium ChannelsPotassium ChannelsElectrical Excitability
