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Journal
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Immunology and Infection
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禽流感监测与FTA卡:实地方法,生物安全,和运输问题解决了
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Please note that all translations are automatically generated.
Click here for the English version.
禽流感监测与FTA卡:实地方法,生物安全,和运输问题解决了
DOI:
10.3791/2832-v
•
12:09 min
•
August 02, 2011
•
Robert H.S. Kraus
,
Pim van Hooft
,
Jonas Waldenström
,
Neus Latorre-Margalef
,
Ronald C. Ydenberg
3
,
Herbert H.T. Prins
1
Resource Ecology Group
,
Wageningen University
,
2
Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences
,
Linnaeus University
,
3
Centre for Wildlife Ecology
,
Simon Fraser University
Chapters
00:05
Title
02:17
Duck Trapping and Cloacal Swabbing
04:02
Viral RNA Isolation and RT-PCR
07:28
Screening for AI-positive Samples and Purification of Targeted Fragments from Gel
08:40
Screening and Identification of PCR Products
09:21
Representative Results
10:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
维护,检测和序列禽流感病毒的RNA的方法进行了验证和扩展,从鸟类的自然粪便样本。这种技术消除了保持冷静的链和处理传染性病毒的必要性和可应用在一个96孔高通量设置。
Tags
Avian Influenza Surveillance
FTA Cards
Field Methods
Biosafety
AIVs
Viral Subtypes
Human Pandemics
H5N1 Outbreaks
Swine Flu Epidemic
Trading And Transportation Activity
Wild Birds
Reservoir Of AIV Strains
Surveillance Programs
Virological Methods
Cold Chains
Biosafety Procedures
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