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Journal / Biology / Erstellung von Qualitäts-Inositol Pyrophosphate
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Erstellung von Qualitäts-Inositol Pyrophosphate
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Erstellung von Qualitäts-Inositol Pyrophosphate

Article DOI: 10.3791/3027-v • 10:35 min • September 3rd, 2011
September 3rd, 2011 •
1, 1, 1, 1, 1
1Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London

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Inositol Pyrophosphate spielen eine wichtige Rolle in der menschlichen Krankheiten wie Krebs, Diabetes und Fettleibigkeit, jedoch ist der genaue Wirkungsmechanismus umstritten. Das Fehlen von kommerziell erhältlichen Inosit Pyrophosphate macht detaillierte Studien problematisch. Hier beschreiben wir ein einfaches Protokoll, zu produzieren und zu isolieren Milligramm Inosit Pyrophosphate.

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Molecular Biology Ausgabe 55 Polyacrilamyde Gel-Elektrophorese (PAGE) Inositol hexakisphosphate (IP6) Phytinsäure diphosphoinositol pentakisphosphate (IP7) bisdiphoshoinositol tetrakisphosphate (IP8) IP6-Kinase (IP6K) PP-IP5K VIP1
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