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Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes
JoVE Journal
Biology
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JoVE Journal Biology
Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes
DOI:

07:33 min

December 08, 2020

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Chapters

  • 00:04Introduction
  • 01:25Assembling the First Monolayer Coated Slide
  • 04:02Laser Irradiation
  • 05:30Results: Analysis of the tBLM Model s Alignment and Measurement of its Corresponding Membrane Conductance Arising from Laser Illumination
  • 06:47Conclusoin

Summary

Automatic Translation

This work outlines a protocol to achieve dynamic, non-invasive monitoring of heat transfer from laser-irradiated gold nanoparticles to tBLMs. The system combines impedance spectroscopy for the real-time measurement of conductance changes across the tBLMs, with a horizontally focused laser beam that drives gold nanoparticle illumination, for heat production.

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