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Journal / Biology / Isolation of CD133+ Liver Stem Cells for Clonal…
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Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Article DOI: 10.3791/3183-v • 12:07 min • October 10th, 2011
October 10th, 2011 •
1, 1, 1, 2, 3
1Department of Pediatrics and Pharmacology, Pennsylvania State College of Medicine, 2Department of Pharmacology, Pennsylvania State College of Medicine, 3Department of Pediatrics, University of California Los Angeles, School of Medicine

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Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.

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CD133+ Liver Stem Cells Clonal Expansion Chronic Liver Injury Hepatocytes Cholangiocytes Liver Cancer Hepatocellular Carcinoma Stem Cell Isolation Solid Organ Rare Population Of Cells Parenchymal Fraction Non-parenchymal Fraction CD45 Negative Cells Enrichment Stem Cell Proliferation Immunohistochemistry Flow Cytometry Core
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