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Rilevamento e Genogrouping di Norovirus dalle feci dei bambini By Taqman One-step RT-PCR
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Rilevamento e Genogrouping di Norovirus dalle feci dei bambini By Taqman One-step RT-PCR

Article DOI: 10.3791/3232-v • 15:17 min • July 22nd, 2012
July 22nd, 2012 •
1, 1, 1,2, 3, 3, 1, 1, 2, 1, 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

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Un One-Step RT-PCR per il rilevamento e l'identificazione genogruppo di isolati Norovirus da feci bambini, che utilizza primer e sonde TaqMan specifiche al telaio di lettura aperta 1 (ORF1)-ORF2 regione di giunzione, la regione più conservata del genoma Norovirus è descritto. Un non-commerciale e conveniente metodo di estrazione dell'RNA è dettagliato.

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Virologia medicina genetica norovirus gastroenteriti estrazione di RNA diarrea campioni di feci PCR RT-PCR TaqMan silice
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