In the following sections, we outline procedures for the preparation of alginate microspheres for use in biomedical applications. We specifically illustrate a technique for creating multilayered alginate microspheres for the dual purpose of cell and protein encapsulation as a potential treatment for type 1 diabetes.
Khanna, O., Larson, J. C., Moya, M. L., Opara, E. C., Brey, E. M. Generation of Alginate Microspheres for Biomedical Applications. J. Vis. Exp. (66), e3388, doi:10.3791/3388 (2012).