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Chapters
- 00:05Title
- 02:02Tissue Preparation
- 03:40Matrix Application
- 05:24MALDI MS Data Acquisition, Processing, and Evaluation
- 12:29Neuropeptide Validation
- 14:18Representative MALDI-MS Results
- 15:55Conclusion
Dopamin erstatning farmakoterapi ved hjelp av L-DOPA er den mest brukte symptomatisk behandling av Parkinsons sykdom, men er ledsaget av bivirkninger, inkludert ufrivillige unormale bevegelser, kalt dyskinesi<sup> 1</sup>. Her er en protokoll for MALDI bildebehandling massespektrometri presentert som registrerer endringer i rottehjernen neuropeptid nivåer relatert til dyskinesier.
Tags
MALDI Imaging Mass SpectrometryNeuropeptidesParkinson’s DiseaseIMSMolecular SpeciesMALDI MatrixIntact PeptidesProteinsMass AnalyzerMALDI TOF/TOF Mass SpectrometerImaging TechniquesMolecules Of InterestUnsupervised AnalysisMolecular SpecificitySensitivityRegion-specific Distribution ProfilesAnimal Model Parkinson’s DiseaseNeurodegenerative DiseaseDopamine ReplacementL-DOPA-induced Dyskinesias (LID)Opioid Precursor Prodynorphin MRNADynorphin PeptidesNeurotransmission
