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Journal / Biology / Pull-down da Calmodulina Proteínas de Ligação
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Pull-down da Calmodulina Proteínas de Ligação
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Pull-down da Calmodulina Proteínas de Ligação

Article DOI: 10.3791/3502-v • 07:52 min • January 23rd, 2012
January 23rd, 2012 •
1, 1, 1, 1
1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

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Calmodulina (CaM) ensaio de pull-down é uma maneira eficaz para investigar a interação de CaM com várias proteínas. Este método usa CaM-sepharose contas para análise eficiente e específica do CaM proteínas de ligação. Isto proporciona uma importante ferramenta para explorar sinalização CaM em função celular.

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Biologia molecular Calmodulina cálcio IQ motif cromatografia de afinidade pull-down Ca2 + / calmodulina quinase II dependente neurogranin
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