This content is Free Access.
Chapters
- 00:05Title
- 01:08Dextran Injection
- 01:53Dissection and Mounting in Unfixed Samples
- 03:13Fixation and Pigmentation Removal
- 05:02Labeling with Alkaline Phosphatase
- 06:02DBA Staining
- 06:49Results: Proximal Convoluted Tubule (PCT) Visualization
- 08:16Conclusion
The zebrafish adult kidney is an excellent system for renal regeneration and disease studies. An essential aspect of such research is the assessment of nephron structure and function. This protocol describes several methodologies that can be implemented to assess nephron tubule composition and to evaluate renal reabsorption.
