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Journal
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Neuroscience
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整体动物灌注固定鼠害
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Whole Animal Perfusion Fixation for Rodents
Please note that all translations are automatically generated.
Click here for the English version.
整体动物灌注固定鼠害
DOI:
10.3791/3564-v
•
08:53 min
•
July 30, 2012
•
Gregory J. Gage
,
Daryl R. Kipke
,
William Shain
1
Biomedical Engineering
,
University of Michigan
,
2
Department of Neurological Surgery
,
University of Washington School of Medicine
Chapters
00:05
Title
01:12
Prepare Apparatus
02:58
Perfusion Surgery
05:08
Perfusion
06:28
Brain Dissection and Storage
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里，我们描述了一个低成本，快速，控制和统一的固定程序，用4％多聚甲醛灌注，通过血管系统：通过对大鼠的心脏，大脑获得尽可能最好的保存。
Tags
Whole Animal Perfusion Fixation
Rodents
Fixation
Immersion Fixation
Hypoxia
Perfusing Fixative
Circulatory System
Vascular Network
Physiological Pressures
Perfusion Fixation Techniques
Paraformaldehyde Perfusion
Brain Preservation
Immunohistochemistry
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