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Ekspression Analyse af mammale Linker-histon Undertyper

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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Beskriver vi en række assays til at analysere ekspressionsniveauer af H1 linker histoner. mRNA individuelle H1 gener kvantitativt ved tilfældig primer baseret revers transkription efterfulgt af real-time PCR, hvorimod protein kvantificering af H1 histoner opnås ved HPLC-analyse.

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Genetik H1 linker histoner og histon H1 subtyper chromatin og RT-PCR HPLC genekspression
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