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JoVE Journal Biology
Isolering af intakt, hele musen mælkekirtlerne for analyse af ekstracellulære Matrix udtryk og morfologi, kirtel
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Isolering af intakt, hele musen mælkekirtlerne for analyse af ekstracellulære Matrix udtryk og morfologi, kirtel

Article DOI: 10.3791/56512
October 30th, 2017 •
1, 2, 2
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 2Department of Biomedical Sciences, GA-PCOM

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October 30th, 2017

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Vi præsenterer her, en protokol til isolering af hele, intakt mus mælkekirtlerne for at undersøge ekstracellulære matrix (ECM) udtryk og duktalt morfologi. Musen #4 abdominal kirtler blev udvundet fra 8-10 uge gammel kvinde har født mus, fast i neutral bufferet formalin, snit og farves ved hjælp af Immunhistokemi for ECM proteiner.

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