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Neuroscience
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小鸡Statoacoustic神经节和脊髓植在胶原凝胶夹层和文化突起生长实验
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
Please note that all translations are automatically generated.
Click here for the English version.
小鸡Statoacoustic神经节和脊髓植在胶原凝胶夹层和文化突起生长实验
DOI:
10.3791/3600-v
•
11:08 min
•
December 20, 2011
•
Kristen N. Fantetti
,
Donna M. Fekete
1
Department of Biological Sciences
,
Purdue University
Chapters
01:10
Bead Preparation
01:52
Statoacoustic Ganglion (SAG) Dissection
03:20
Spinal Cord Dissection
05:28
The Explant Culture with Either Purified Proteins or Protein-coated Beads
07:53
Visualizing of Neurite Outgrowth by Immunohistochemistry
09:02
Representative Results from Neurite Outgrowth Assays
10:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们演示了如何解剖和文化小鸡E4 statoacoustic节和E6脊髓植。外植体无血清条件下24小时的三维胶原凝胶培养。突起的响应测试与生长因子补充介质与蛋白包被的磁珠。
Tags
Dissection
Culture
Chick Statoacoustic Ganglion
Spinal Cord Explants
Collagen Gels
Neurite Outgrowth Assays
Sensory Organs
Inner Ear
SAG Neurons
Axon Guidance Cues
Survival Factors
Otic Axons Misrouting
Growth Factors
Neurotrophin-3 (NT-3)
Brain-derived Neurotrophic Factor (BDNF)
Transgenic Animals
In Vitro Method
Responsiveness Of SAG Neurites
Soluble Proteins
Morphogens
Neuron Survival
Embryonic Day 4 (E4)
Three-dimensional Collagen Gels
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