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Journal / Bioengineering / Site-spezifische bakterielle Chromosom…
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Bioengineering

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JoVE Journal Bioengineering
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Site-spezifische bakterielle Chromosom Engineering: ΦC31 Integrase vermittelte Cassette Exchange (IMCE)

Article DOI: 10.3791/3698-v • 08:21 min • March 16th, 2012
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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Eine schnelle und effiziente Methode, um Fremd-DNA von Interesse in vorgefertigte Akzeptor Stämme, sogenannte Landing-Pad-Stämme, die Integration beschrieben wird. Das Verfahren erlaubt eine ortsspezifische Integration eines DNA-Kassette in die gentechnisch Landeplatz Ort einer gegebenen Dehnung, durch Konjugation und Expression des ΦC31 Integrase.

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Bioengineering ΦC31 Integrase Rhizobiales Chromosome Engineering Bakteriengenetik
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