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JoVE Journal Neuroscience
Multiphoton Mikroskopi af Cleared Mouse Brain udtrykker YFP
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Multiphoton Mikroskopi af Cleared Mouse Brain udtrykker YFP

Article DOI: 10.3791/3848-v • 10:04 min • September 23rd, 2012
September 23rd, 2012 •
*1, *1, 1,2, 1
1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University
* These authors contributed equally

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Multiphoton microscopy af hele muse organer er mulig ved optisk fjerne organ før billeddannelse, men ikke alle protokoller bevare det fluorescerende signal af fluorescerende proteiner. Brug af et optisk clearing metode med ethanol-baseret dehydrering og benzylalkohol: benzylbenzoat clearing, viser vi i høj opløsning multiphoton billeder af hele musehjerne udtrykker YFP.

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Neuroscience Biomedical Engineering Molekylærbiologi multiphoton mikroskopi mus hjerne clearing YFP fluroescence
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