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Chapters
- 00:04Title
- 00:37Microscope Design: Assembly of the Excitation Path
- 02:18Microscope Design: Assembly of the Emission Path
- 02:59Diffraction-limited Imaging with Epi-excitation
- 03:36Multi-channel Single-molecule Imaging Including smFRET
- 05:37SR Imaging
- 06:59Results: Multiple High Resolution Imaging Modes on a Single Fluorescence Microscope
- 08:11Conclusion
Here we present a practical guide of building an integrated microscopy system, which merges conventional epi-fluorescent imaging, single-molecule detection-based super-resolution imaging, and multi-color single-molecule detection, including single-molecule fluorescence resonance energy transfer imaging, into one set-up in a cost-efficient way.
