Conducting Multiple Imaging Modes with One Fluorescence Microscope

This method can help answer key questions in the optical microscopy field, such as how to take super-resolution images as well as FRET images using the same microscope. The main advantage of this technique is that we could combine three different imaging modules into one microscope, significantly reducing the overall cost. Visual demonstration of this method is critical as assembly of the excitation path is difficult to learn.

They require proper choice of parts and a sensitive optical alignments. To begin, install a data acquisition card through a PCI interface and use it to connect the lasers to the computer. Control the lasers'on and off behaviors by transistor-transistor logic output and their power adjustment by the analog output of this card.