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JoVE Journal
Immunology and Infection

This content is Open Access.

检测使用荧光脱氧核酶的细菌
 

检测使用荧光脱氧核酶的细菌

Article doi: 10.3791/3961
May 28th, 2012

Summary May 28th, 2012

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我们最近报道一种新的方法,产生荧光脱氧核酶探针,可用于设立一个简单的“混合和阅读的”细菌检测荧光法。这些特殊的DNA探针,催化裂解1生色修饰DNA-RNA的存在原油外的一个特定的细菌,从而转化为细菌检测荧光信号的产生,产生的混合物(CEM)的嵌合基板。在这份报告中,我们将描述模型的细菌检测采用特定的核酶探针记“RFD的EC1的”为重点的实验程序,

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