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Journal / Immunology and Infection / 检测使用荧光脱氧核酶的细菌
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
检测使用荧光脱氧核酶的细菌
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检测使用荧光脱氧核酶的细菌

Article DOI: 10.3791/3961-v • 13:20 min • May 28th, 2012
May 28th, 2012 •
1, 1, 1, 1,2
1Department of Biochemistry and Biomedical Sciences, McMaster University, 2Department of Chemistry and Chemical Biology, McMaster University

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我们最近报道一种新的方法，产生荧光脱氧核酶探针，可用于设立一个简单的“混合和阅读的”细菌检测荧光法。这些特殊的DNA探针，催化裂解1生色修饰DNA-RNA的存在原油外的一个特定的细菌，从而转化为细菌检测荧光信号的产生，产生的混合物（CEM）的嵌合基板。在这份报告中，我们将描述模型的细菌检测采用特定的核酶探针记“RFD的EC1的”为重点的实验程序，

Tags

生物化学，63期，免疫荧光脱氧核酶，大肠杆菌，生物传感器，检测细菌
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