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FRET显微镜在活细胞单分子生物传感器的信号事件的实时监测
JoVE Journal
Biology
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JoVE Journal Biology
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
DOI:

10:34 min

August 20, 2012

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Chapters

  • 00:05Title
  • 01:38FRET Imaging Microscope Assembly
  • 03:34Imaging Software Installation and Configuration
  • 05:37Live-cell Imaging with FRET
  • 08:35Monitoring Intracellular cAMP by FRET Imaging
  • 09:42Conclusion

Summary

Automatic Translation

福斯特共振能量转移(FRET)显微镜是一种功能强大的技术,使用各种生物传感器记者在活细胞中的信号转导事件的实时监控。在这里,我们将介绍如何建立一个自定义的表面荧光共振成像系统从商业上可用的组件和如何使用它的FRET实验。

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