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FRET显微镜在活细胞单分子生物传感器的信号事件的实时监测

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Biology

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JoVE Journal Biology

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

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FRET显微镜在活细胞单分子生物传感器的信号事件的实时监测

DOI:

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10:34 min

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August 20, 2012

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Julia U. Sprenger, Ruwan K. Perera, Konrad R. Götz, Viacheslav O. Nikolaev

¹Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen,Georg August University Medical Center, Göttingen, Germany

Chapters

00:05Title
01:38FRET Imaging Microscope Assembly
03:34Imaging Software Installation and Configuration
05:37Live-cell Imaging with FRET
08:35Monitoring Intracellular cAMP by FRET Imaging
09:42Conclusion

Summary

Automatic Translation

福斯特共振能量转移（FRET）显微镜是一种功能强大的技术，使用各种生物传感器记者在活细胞中的信号转导事件的实时监控。在这里，我们将介绍如何建立一个自定义的表面荧光共振成像系统从商业上可用的组件和如何使用它的FRET实验。

Tags

FRET Microscopy Real-time Monitoring Signaling Events Live Cells Unimolecular Biosensors Genetically-encoded Biosensors Protein-protein Interactions Conformational Changes Fluorophore-tagged Proteins Binding Moiety Molecule Of Interest G-protein Activation Second Messengers Epifluorescence FRET Imaging System Micro-Manager Freeware

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