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Chapters
- 00:05Title
- 01:38FRET Imaging Microscope Assembly
- 03:34Imaging Software Installation and Configuration
- 05:37Live-cell Imaging with FRET
- 08:35Monitoring Intracellular cAMP by FRET Imaging
- 09:42Conclusion
Förster resonans (FRET) mikroskopi er en kraftfuld teknik til real-time overvågning af signalering begivenheder i levende celler ved hjælp af forskellige biosensorer som reportere. Her beskriver vi, hvordan man opbygger et tilpasset epifluorescens FRET imaging system fra kommercielt tilgængelige komponenter, og hvordan man bruger det til FRET eksperimenter.
Tags
FRET MicroscopyReal-time MonitoringSignaling EventsLive CellsUnimolecular BiosensorsGenetically-encoded BiosensorsProtein-protein InteractionsConformational ChangesFluorophore-tagged ProteinsBinding MoietyMolecule Of InterestG-protein ActivationSecond MessengersEpifluorescence FRET Imaging SystemMicro-Manager Freeware
