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膜片钳记录在内耳毛细胞的分离斑马鱼
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
膜片钳记录在内耳毛细胞的分离斑马鱼
DOI:
10.3791/4281-v
•
12:43 min
•
October 17, 2012
•
Rachel Einarsson
,
Marshall Haden
,
Gabrielle DiCiolli
,
Andrea Lim
,
Kolina Mah-Ginn
,
Kathleen Aguilar
,
Lucy Yazejian
,
Bruce Yazejian
1
Natural Science Division
,
Pepperdine University
Chapters
00:05
Title
01:52
Pre-experimental Preparations
02:47
Isolation of Auditory and Vestibular Labyrinth
05:28
Hair Cell Isolation
07:18
Patch Clamping Zebrafish Hair Cells
10:12
Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
12:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频的目的是为了证明程序获得的成年斑马鱼的内耳器官的健康,完整的毛细胞,然后利用他们为特征的生物物理特性的电压门控离子通道的膜片钳研究的目的。
Tags
Patch Clamp Recordings
Inner Ear Hair Cells
Zebrafish
Voltage-gated Channels
Sensory Hair Cells
Isolation Method
Hair Cell Size
Hair Cell Morphology
Diversity
Patch Clamp Recordings
Zebrafish Model System
Mutants
Hearing And Balance
Transgenic Lines
Genetic Analysis
Electrophysiological Methods
Sensory Modalities
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