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时间推移荧光成像拟南芥根系生长的根环境的快速操作使用RootChip
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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时间推移荧光成像拟南芥根系生长的根环境的快速操作使用RootChip

DOI:
10.3791/4290-v

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13:54 min

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July 07, 2012

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, , , , , ,
1Department of Plant Biology,Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering,Stanford University , 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS),University of Freiburg

Chapters

  • 00:05Title
  • 03:24Step 1: Preparation of Growth Cones for Seed Germination
  • 03:58Step 2: Seed Germination and Seedling Growth
  • 05:02Step 3: Transfer of Seedlings onto the RootChip
  • 06:51Step 4: Connecting the RootChip to the Carrier
  • 08:11Step 5: Mounting the RootChip at the Microscope
  • 09:18Step 6: Operating the RootChip using the LabView Interface
  • 11:09Results
  • 12:43Conclusion

Summary

Automatic Translation

Automatic Translation

本文提供中的RootChip的拟南芥幼苗栽培的协议，一个微成像平台，结合微观根监测和基于FRET的细胞代谢水平的测量与自动化控制生长条件。

Tags

Time-lapseFluorescence ImagingArabidopsis Root GrowthRootChipRoot Environment ManipulationMicrofluidic DeviceNutrient UptakeSustainable ApproachesCrop YieldMicroscopic DetailPolydimethylsiloxane (PDMS)Micromechanical ValvesPerfusion SystemObservation ChambersTest SolutionRoot Biology

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