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使用一个荧光显微镜进行多种成像模式

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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使用一个荧光显微镜进行多种成像模式

DOI:

10.3791/58320-v

•

08:32 min

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October 28, 2018

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Seongjin Park, Jiacheng Zhang, Matthew A. Reyer, Joanna Zareba³, Andrew A. Troy, Jingyi Fei²

¹Department of Biochemistry and Molecular Biology,University of Chicago, ²The Institute for Biophysical Dynamics,University of Chicago, ³Faculty of Chemistry,Wrocław University of Science and Technology, ⁴Nikon Instruments Inc.

Chapters

00:04Title
00:37Microscope Design: Assembly of the Excitation Path
02:18Microscope Design: Assembly of the Emission Path
02:59Diffraction-limited Imaging with Epi-excitation
03:36Multi-channel Single-molecule Imaging Including smFRET
05:37SR Imaging
06:59Results: Multiple High Resolution Imaging Modes on a Single Fluorescence Microscope
08:11Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个实用的指南, 建立一个综合显微镜系统, 合并传统的 epi 荧光成像, 单分子检测的超分辨率成像和多色单分子检测, 包括单分子荧光共振能量转移成像, 成一种以经济高效的方式进行设置。

Tags

Fluorescence Microscopy Imaging Modes Super-resolution Imaging FRET Imaging Multicolor Imaging Single-molecule FRET Data Acquisition Optical Alignment Vibration Isolation Laser Excitation Emission Filters Epifluorescence Diffraction-limited Imaging

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