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JoVE Journal Biology
评估肾细胞​​蛋白激酶C同工酶，线粒体功能及细胞活力的
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
Journal (Biology)
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Journal (Biology)
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
 
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评估肾细胞​​蛋白激酶C同工酶，线粒体功能及细胞活力的

Article DOI: 10.3791/4301
January 7th, 2013 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences

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January 7th, 2013

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活化的蛋白激酶C（PKC）的同工酶与呼吸，氧化磷酸化有关的线粒体功能和对细胞活力的影响进行说明。该方法适应腺病毒技术，选择性过度表达PKC同工酶在原代培养和各种检测，以确定线粒体功能和能量状态的细胞。

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