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Vurdering af Mitochondriale funktioner og cellelevedygtighed i Renal celler, der overudtrykker protein kinase C isozymer
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Vurdering af Mitochondriale funktioner og cellelevedygtighed i Renal celler, der overudtrykker protein kinase C isozymer

Article DOI: 10.3791/4301 • 15:43 min
January 7th, 2013 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences

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January 7th, 2013

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Virkningerne af aktivering af proteinkinase C (PKC) isozymer på mitochondriale som er forbundet med åndedræt og oxidativ phosphorylering og på cellelevedygtighed, er beskrevet. Den fremgangsmåde tilpasser adenoviral teknik til selektivt at overudtrykke PKC-isozymer i primær cellekultur og en række assays til bestemmelse mitochondriale funktioner og energi status af cellen.

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