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定量荧光共振能量转移(福斯特共振能量转移)SENP1蛋白酶的动力学测定分析
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination
DOI:

16:02 min

February 21, 2013

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Chapters

  • 00:05Title
  • 02:20Plasmid Constructs
  • 03:07Protein Expression and Purification
  • 06:16FRET-based Protease Assay for Enzyme Kinetic Study
  • 08:54Quantitative FRET Spectrum Analysis
  • 13:36Representative Results
  • 15:11Conclusion

Summary

Automatic Translation

涉及的FRET(福斯特共振能量转移)信号的定量分析酶动力学研究的一种新方法。<em> K<sub> M</sub</em>和<em> K<sub>猫</sub</em> SENP1(SUMO / Sentrin特定的蛋白酶1)预SUMO1(小泛素样修饰)的催化结构域的水解获得。这个定量的基于FRET的蛋白酶动力学研究的一般原则可以应用到其他的蛋白酶。

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