Name: Propagation of Human Embryonic Stem (ES) Cells (Video) | JoVE Journal
Uploaded: 2006-11-30T00:00:00.000+00:00
Duration: 12 min 52 s
Description: Learn to propagate human embryonic stem cells using collagenase splitting and colony picking techniques. This method maintains H1 and H9 cell lines for regenerative medicine and tissue engineering research.

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<ol><li>Kawasaki, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Induction+of+midbrain+dopaminergic+neurons+from+ES+cells+by+stromal+cell-derived+inducing+activity%5BTitle%5D)+AND+%22Neuron%22%5BJournal%5D)">Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.</a> Neuron. 28, 31-40 (2000).</li>
<li>Perrier, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Derivation+of+midbrain+dopamine+neurons+from+human+embryonic+stem+cells%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Derivation of midbrain dopamine neurons from human embryonic stem cells.</a> Proc Natl Acad Sci U S A. 101, 12543-12548 (2004).</li>
<li>Pruszak, J., Isacson, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Directed+differentiation+of+human+embryonic+stem+cells+into+dopaminergic+neurons%5BTitle%5D)+AND+%22%22%5BJournal%5D)">Directed differentiation of human embryonic stem cells into dopaminergic neurons.</a> , John Wiley and Sons. (2007).</li>
<li>Pruszak, J., Sonntag, K. C., Aung, M. H., Sanchez-Pernaute, R., Isacson, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Markers+and+methods+for+cell+sorting+of+human+embryonic+stem+cell-derived+neural+cell+populations%5BTitle%5D)+AND+%22Stem+Cells%22%5BJournal%5D)">Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.</a> Stem Cells. 25, 2257-2268 (2007).</li>
<li>Sonntag, K. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Enhanced+yield+of+neuroepithelial+precursors+and+midbrain-like+dopaminergic+neurons+from+human+embryonic+stem+cells+using+the+bone+morphogenic+protein+antagonist+noggin%5BTitle%5D)+AND+%22Stem+Cells%22%5BJournal%5D)">Enhanced yield of neuroepithelial precursors and midbrain-like dopaminergic neurons from human embryonic stem cells using the bone morphogenic protein antagonist noggin.</a> Stem Cells. 25, 411-418 (2007).</li>
<li>Kawasaki, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Generation+of+dopaminergic+neurons+and+pigmented+epithelia+from+primate+ES+cells+by+stromal+cell-derived+inducing+activity%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity.</a> Proc Natl Acad Sci U S A. 99, 1580-1585 (2002).</li>
<li>Ko, J. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+embryonic+stem+cell-derived+neural+precursors+as+a+continuous%2C+stable%2C+and+on-demand+source+for+human+dopamine+neurons%5BTitle%5D)+AND+%22J+Neurochem%22%5BJournal%5D)">Human embryonic stem cell-derived neural precursors as a continuous, stable, and on-demand source for human dopamine neurons.</a> J Neurochem. 103, 1417-1429 (2007).</li>
<li>Hong, S., Kang, U. J., Isacson, O., Kim, K. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neural+precursors+derived+from+human+embryonic+stem+cells+maintain+long-term+proliferation+without+losing+the+potential+to+differentiate+into+all+three+neural+lineages%2C+including+dopaminergic+neurons%5BTitle%5D)+AND+%22J+Neurochem%22%5BJournal%5D)">Neural precursors derived from human embryonic stem cells maintain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons.</a> J Neurochem. , (2007).</li>
<li>Zhang, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neural+subtype+specification+from+embryonic+stem+cells%5BTitle%5D)+AND+%22Brain+Pathol%22%5BJournal%5D)">Neural subtype specification from embryonic stem cells.</a> Brain Pathol. 16, 132-142 (2006).</li>
<li>Pankratz, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Directed+neural+differentiation+of+human+embryonic+stem+cells+via+an+obligated+primitive+anterior+stage%5BTitle%5D)+AND+%22Stem+Cells%22%5BJournal%5D)">Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.</a> Stem Cells. 25, 1511-1520 (2007).</li>
<li>Lazzari, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Direct+derivation+of+neural+rosettes+from+cloned+bovine+blastocysts%3A+a+model+of+early+neurulation+events+and+neural+crest+specification+in+vitro%5BTitle%5D)+AND+%22Stem+Cells%22%5BJournal%5D)">Direct derivation of neural rosettes from cloned bovine blastocysts: a model of early neurulation events and neural crest specification in vitro.</a> Stem Cells. 24, 2514-2521 (2006).</li>
<li>Pomp, O., Brokhman, I., Ben-Dor, I., Reubinoff, B., Goldstein, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Generation+of+peripheral+sensory+and+sympathetic+neurons+and+neural+crest+cells+from+human+embryonic+stem+cells%5BTitle%5D)+AND+%22Stem+Cells%22%5BJournal%5D)">Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells.</a> Stem Cells. 23, 923-930 (2005).</li>
</ol>

propagation of human embryonic stem (es) cells

Propagation of Human Embryonic Stem (ES) Cells

Yeah, so I'm Lauren Deon. I'm in charge of the Human Amgen stem cell co facility at MGH. Yeah, so I'm Lauren, I'm in charge of the Human AM stem cell co facility at MGH.So I've been working with human Amgen stem cells for years now since I was a postdoc in George De Lab before, from four, four years. And so right now we have two cell lines, H one and H nine, which are both from Y cell. And so the way we are expanding or maintaining these cells in culture is by, at least the way we are splitting these cells is by using collagenous.So what I usually do is for the first few passage after throwing the cells, I use a technical, the picking colonies. So in this case, I can check under the microscope which colonies are good and differentiate and nice shape, and I will collect only these colonies and I will cut them in few pieces and put them back in, in a fresh flat. And the, the colonies that look kind of differentiate it are just left them alone.The other way to pass the cells is by using trypsin. So I don't use this technique here. I think collagenase is a little bit safer because human being stem cells don't like to be individualized or they don't like to be alone.So if you do trypsin

propagation-of-human-embryonic-stem-es-cells

Research

JoVE Journal

Biology

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Derivation of Human Embryonic Stem Cells by Immunosurgery

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Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation 

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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

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Freezing Human ES Cells

Learn to propagate human embryonic stem cells using collagenase splitting and colony picking techniques. This method maintains H1 and H9 cell lines for regenerative medicine and tissue engineering research.

Propagation of Human Embryonic Stem (ES) Cells

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