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Biology
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活细胞成像早期自噬活动:Omegasomes与超越
JoVE Journal
Biology
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JoVE Journal
Biology
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Please note that all translations are automatically generated.
Click here for the English version.
活细胞成像早期自噬活动:Omegasomes与超越
DOI:
10.3791/50484-v
•
09:00 min
•
July 27, 2013
•
Eleftherios Karanasios
,
Eloise Stapleton
,
Simon A. Walker
,
Maria Manifava
,
Nicholas T. Ktistakis
1
Signalling Programme
,
The Babraham Institute
,
2
MRC Group, Cardiff School of Biosciences
,
Cardiff University
Chapters
00:05
Title
01:17
Preparing Cells
02:59
Preparing the Incubation Chamber for Live Cell Imaging
04:04
Microscopy
05:22
Results: LC3-positive Autophagosomes Originate from DFCP1-positive Omegasomes
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
时间推移显微镜荧光标记的细胞自噬标记允许自噬反应具有高时空分辨率的动态监测。使用特定的自噬和细胞器标记在3种不同颜色的组合,我们可以按照自噬体形成在一个强大的空间和时间上下文的蛋白质的贡献。
Tags
Autophagy
Autophagosomes
Omegasomes
Live Cell Imaging
DFCP1
Protein Of Interest
Membrane Trafficking
Lysosome
Mitochondria
Time-lapse Microscopy
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