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Immunology and Infection

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JoVE Journal Immunology and Infection
Levande cell imaging av Alphaherpes Virus Anterograd Transport och Spread
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Levande cell imaging av Alphaherpes Virus Anterograd Transport och Spread

Article DOI: 10.3791/50723-v • 15:31 min • August 16th, 2013
August 16th, 2013 •
1,2, 2, 2
1Department of Immunology and Infectious Diseases, Montana State University, 2Department of Molecular Biology, Princeton University

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Levande cell imaging av alphaherpes virusinfektioner möjliggör analys av dynamiska händelser riktad transport och intercellulär spridning. Här presenterar vi metoder som utnyttjar rekombinanta virusstammar som uttrycker fluorescerande fusionsproteiner att underlätta visualisering av virala församlingar under infektion av primära neuroner.

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Virologi 78 infektion immunologi medicin molekylärbiologi cellbiologi mikrobiologi genetik mikroskopi fluorescens neurobiologi herpesvirus fluorescerande protein epifluorescent mikroskopi neuronal kultur axon virus video mikroskopi virus levande cell imaging
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