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Journal / Immunology and Infection / Intravital widefield fluorescensmikroskopi av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Intravital widefield fluorescensmikroskopi av lungmikrocirkulation vid experimentell akut lungskada med hjälp av ett vakuumstabiliserat bildsystem
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Intravital widefield fluorescensmikroskopi av lungmikrocirkulation vid experimentell akut lungskada med hjälp av ett vakuumstabiliserat bildsystem

Article DOI: 10.3791/63733-v • 09:28 min • April 6th, 2022
April 6th, 2022 •
1, 2, 2,3, 4, 5, 6,7, 2,4, 1,2,4,8
1Department of Physiology and Biophysics, Dalhousie University, 2Department of Microbiology and Immunology, Dalhousie University, 3Department of Neuroscience, Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, 5Live Cell Imaging Center, University of Calgary, 6Department of Physiology and Pharmacology, University of Calgary, 7Department of Biochemistry and Molecular Biology, University of Calgary, 8Department of Pharmacology, Dalhousie University

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Intravital fluorescensmikroskopi kan användas för att studera leukocyt-endotelinteraktioner och kapillärperfusion i realtid. Detta protokoll beskriver metoder för att avbilda och kvantifiera dessa parametrar i lungmikrocirkulationen med hjälp av ett vakuumstabiliserat lungavbildningssystem.

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Immunologi och infektion utgåva 182
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