Journal
/
Immunology and Infection
/
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

DOI:
10.3791/50723-v

•

15:31 min

•

August 16, 2013

•

, ,
1Department of Immunology and Infectious Diseases,Montana State University, 2Department of Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 01:27Environmental Control Conditions for Live-cell Fluorescent Microscopy
  • 03:44Real-time Imaging of Anterograde Virion Transport Events
  • 06:43Overnight Time-lapse Imaging of Anterograde Spread Events
  • 09:08Image Processing and Export
  • 10:48Results: Representative Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
  • 15:06Conclusion

Summary

Automatic Translation

Automatic Translation

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

Tags

Anterograde TransportLive Cell ImagingFluorescent Fusion ProteinsViral Particle AssembliesCell-cell SpreadParticle Transport VelocitiesProtein LocalizationViral Genetic TechniquesAlphaherpes Virus

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50723?article_type=v

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
check_url/50723?article_type=v

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
check_url/50723?article_type=v

Real-time Live Imaging of T-cell Signaling Complex Formation
check_url/50723?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/50723?article_type=v

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
check_url/50723?article_type=v

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
check_url/50723?article_type=v

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
check_url/50723?article_type=v

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
check_url/50723?article_type=v

Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System
check_url/50723?article_type=v

Virus Propagation and Cell-Based Colorimetric Quantification

Read Article