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Chapters
- 00:05Title
- 01:27Plate-bound anti-CD3
- 02:03Mouse Dissection
- 04:40Th17 Differentiation
- 05:40qPCR and ELISA
- 06:27Cell Activation
- 07:09Intracellular Staining
- 08:55Flow Cytometric Analysis
- 09:24Results: Isolation and Differentiation of Th17 Cells
- 12:14Conclusion
Med effektorfunktioner adskiller sig fra andre T-celle delmængder, har Th17 celler blevet centralt impliceret i inflammatoriske autoimmunitet. Denne in vitro Th17 differentiering protokol giver et middel til at afgøre, om naive CD4 + T-lymfocytter kan differentiere sig Th17 celler, og for yderligere at undersøge deres rolle i autoimmunitet og vært reaktion.
