Isolation and Ex Vivo Culture of V&#948;1+CD4+&#947;&#948; T Cells, an Extrathymic &#945;&#946;T-cell Progenitor

Christian Welker; Rupert Handgretinger; Karin Schilbach

doi:10.3791/53482

Journal

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Immunology and Infection

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Isolation and Ex Vivo Culture of Vδ1⁺CD4⁺γδ T Cells, an Extrathymic αβT-cell Progenitor

Isolation and <em>Ex Vivo</em> Culture of Vδ1<sup>+</sup>CD4<sup>+</sup>γδ T Cells, an Extrathymic αβT-cell Progenitor

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Ex Vivo Culture of Vδ1⁺CD4⁺γδ T Cells, an Extrathymic αβT-cell Progenitor

Isolation and Ex Vivo Culture of Vδ1⁺CD4⁺γδ T Cells, an Extrathymic αβT-cell Progenitor

9,296 Views

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10:33 min

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December 07, 2015

DOI:

10.3791/53482-v

DOI:

10.3791/53482-v

•

10:33 min

December 07, 2015

•

9279 Views

Christian Welker¹, Rupert Handgretinger¹, Karin Schilbach¹

¹Deptartment of Hematology and Oncology,Children’s University Hospital Tübingen

Summary

Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1⁺CD4⁺ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.