Journal
/
/
Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor
Isolation and <em>Ex Vivo</em> Culture of Vδ1<sup>+</sup>CD4<sup>+</sup>γδ T Cells, an Extrathymic αβT-cell Progenitor
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

9,296 Views

10:33 min

December 07, 2015

DOI:

10:33 min
December 07, 2015

9279 Views
, ,

Summary

Automatically generated

Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1+CD4+ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.

Related Videos

Read Article