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Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.
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Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

DOI:
10.3791/50838-v

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10:08 min

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November 20, 2013

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, ,
1Department of Cell and Molecular Biology,Feinberg School of Medicine, Northwestern University, 2IKERBASQUE,Basque Foundation for Science

Chapters

  • 00:05Title
  • 01:34Drosophila S2 Cells
  • 03:19Drosophila Neuron Culture
  • 07:47Visualized Microtuble Transport in Cultured S2 Cells and Neurons
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.

Tags

DrosophilaS2 CellsPrimary NeuronsOrganelle TransportMicrotubulesLive ImagingRNAiCargo TransportAxonal TransportCell Culture

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